亚洲国产精品精华-亚洲国产精品色一区二区-亚洲国产精品无码av-亚洲国产精品无码成人A片小说-亚洲国产精品无码成人片久久-亚洲国产精品无码观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2888WPE-int 人正常前列腺上皮細(xì)胞

WPE-int 人正常前列腺上皮細(xì)胞

簡要描述:CRL-2888 WPE-int 人正常前列腺上皮細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種原代細(xì)胞、細(xì)胞系。細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2888
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:1391

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2888 WPE-int 人正常前列腺上皮細(xì)胞

ATCC® Number:CRL-2888™    Price:$329.00
Designations:WPE-int

Depositors:MM Webber, EJ Tokar

Biosafety Level:2 [cells contain human HPV-18 viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial
WPE-int 人正常前列腺上皮細(xì)胞


Source:Organ: prostate, normal, peripheral zone
Cell Type: epithelial


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2888 WPE-int 人正常前列腺上皮細(xì)胞

Isolation:Isolation date: 2004 [PubMed: 16351690] [92794]

Receptors:androgen receptor, expressed ( [92794] upregulated upon exposure to androgen)

Tumorigenic:NO

Antigen Expression:kallikrein 3 ( KLK3); prostate specific antigen (PSA); expressed (upregulated upon exposure to androgen) [PubMed: 16351690] [92794]

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 13

D13S317: 8,14

D16S539: 9,11

D5S818: 12,15

D7S820: 10,11

THO1: 8

TPOX: 8,11

vWA: 14,18



Age:54

Gender:male

Ethnicity:Caucasian

Comments:WPE-int cells were derived from the RWPE-1 cell line (ATCC CRL-11609) after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line (ATCC CRL-2887). They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line (ATCC CRL-2887), which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.
  1. The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. (personal communication from depositor).



Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit

  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.




CRL-2888

ATCC® Number: CRL-2888™ Price: $329.00
Designations: WPE-int
Depositors: MM Webber, EJ Tokar
Biosafety Level: 2 [cells contain human HPV-18 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial


Source: Organ: prostate, normal, peripheral zone
Cell Type: epithelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 2004 [PubMed: 16351690] [92794]
Receptors: androgen receptor, expressed ( [92794] upregulated upon exposure to androgen)
Tumorigenic: NO
Antigen Expression: kallikrein 3 ( KLK3); prostate specific antigen (PSA); expressed (upregulated upon exposure to androgen) [PubMed: 16351690] [92794]
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,14
D16S539: 9,11
D5S818: 12,15
D7S820: 10,11
THO1: 8
TPOX: 8,11
vWA: 14,18
Age: 54
Gender: male
Ethnicity: Caucasian
Comments: WPE-int cells were derived from the RWPE-1 cell line (ATCC CRL-11609) after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line (ATCC CRL-2887). They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line (ATCC CRL-2887), which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.
The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. (personal communication from depositor).
Propagation: ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
•0.05 mg/ml BPE - provided with the K-SFM kit
•5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 亚洲一线二线三线品牌精华液久久久 | 成人爽a毛片免费视频 | 日本黄色免费 | 久久久久久亚洲精品首页 | 91福利国产在线观看香蕉 | 欧美二区三区久本道 | 91麻豆成人精品国产免费网站 | 日本中文字幕巨大的乳专区 | 欧美一区2区三区3区公司 | 精品国产一区二区三 | 久青草免费视频 | 国产精品ⅴa在线观看无码电影 | 顶着薄薄的丝袜进入在 | 少妇无码一区二区三区免费 | 欧美日韩在线永久免费播放 | 亚洲中文字幕人妻 | 久久久久久久精品免费 | 国产无码高清大片 | 911日韩精品影视 | 国产精品自在线国 | 97久久精品国产成人影院 | 91人妻人人做人碰人人爽九色 | 欧美亚洲日韩午夜激情影院 | 2024国产成人精品福利网站 | 久久精品国产欧美日韩亚洲 | 成人毛片在线免费在线 | 国产精品亚洲欧美动漫 | 91精品国产经典在线观看 | 99久久无码精品国产免费 | 亚洲国产成人精品无码素人福利 | 一边吃奶一边做边爱hd在线视频播放 | 国产亚洲精品久久久一区 | 国产成人福利美女观看视频 | 国产a精品区二区三区四区 国产a精品一区二区 | 国产av办公室丝袜秘书 | 国产又粗又长又大A片激情 国产又粗又长又大精品A片 | 国内精品久久久久久无码不卡 | 91精品国产福利在线观看性色 | av无码久久久久不卡蜜桃 | 大片免免费观看视频播放器在线观看 | 亚洲欧美日韩国产精品一区二 |