亚洲国产精品精华-亚洲国产精品色一区二区-亚洲国产精品无码av-亚洲国产精品无码成人A片小说-亚洲国产精品无码成人片久久-亚洲国产精品无码观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-1772C2C12細(xì)胞, 小鼠成肌細(xì)胞

C2C12細(xì)胞, 小鼠成肌細(xì)胞

簡(jiǎn)要描述:C2C12細(xì)胞, 小鼠成肌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1772
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1933

產(chǎn)品分類

Product Category

詳細(xì)介紹

C2C12細(xì)胞, 小鼠成肌細(xì)胞

ATCC® Number:CRL-1772™    Price:$256.00
Designations:C2C12Biosafety Level:1Shipped:frozenMedium Properties:& Serum:oSee PropagationGrowthadherentOrganism:Mus musculus (muse)Morphology:myoblastSource:Strain: C3H Tissue: muscle Cell Type: myoblast;Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. C2C12細(xì)胞, 小鼠成肌細(xì)胞Applications:transfection host (Nucleofection technology from LonzaRoche FuGENE® Transfection Reagents)Comments:This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903] The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953] Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427] Tested and found negative for ectromelia virus (mousepox).Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT. Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.Incubate cultures at 37°C.



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 久草国产在线播放 | 九九精品视频一区二区三区 | 91亚洲精品无码久久久久 | 国产精品亚洲视频在线观看 | 福利一区二区 | 日本一本有码无码综合视频 | 国内精品久久久久久无码不卡 | 精品视自拍视频在线观看 | 成人无码a片在线最新欧美av女人xxxx人猿泰山成人 | 国产av无码不卡 | 精品无码专区第一页 | 日日摸夜夜添夜夜爽出水 | 老湿机69福利 | 亚洲精品无码v专区最新 | 亚洲波霸一区二区在线观看 | 国产激情电影综合在线观看 | 国产+高潮+喷浆 | 六月色婷婷| 亚洲综合久久久久久中文字幕 | 婷婷五月久久丁香国产综合 | 97人人添人人澡人人澡人人澡 | 国产精品国内免费一区二区三区 | 18禁裸乳无遮挡自慰免费 | 国产精品扒开腿做爽爽爽王者A片 | 99久久亚洲精品无码毛片 | 精品人妻无码专区在线视频 | av无码av无码专区 | 精品无码国产自产在线观看极品 | 国产午夜一区二区三 | 国产成人精品毛片曰本亚洲 | 91国内揄拍国内 | 中文字幕无码不卡一区二区三区 | 91精品国产福利在线观 | 91精品国内手机在线观看 | 欧美日韩精品一区二区播放电影 | 99久久精品这里只有精品 | 9久9久女女热精品视频免费观看 | 国产成a人片在线观看视频 国产成a人片在线观看视频99 | 中国性少妇内 | 久久精品免费i国产 | 国产精品国产三级国产av品爱 |