亚洲国产精品精华-亚洲国产精品色一区二区-亚洲国产精品无码av-亚洲国产精品无码成人A片小说-亚洲国产精品无码成人片久久-亚洲国产精品无码观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>HB-82BB7.2小鼠 雜交瘤 B淋巴細(xì)胞

BB7.2小鼠 雜交瘤 B淋巴細(xì)胞

簡要描述:HB-82 BB7.2小鼠 雜交瘤 B淋巴細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞株|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:HB-82
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2151

產(chǎn)品分類

Product Category

詳細(xì)介紹

HB-82 BB7.2小鼠 雜交瘤 B淋巴細(xì)胞 的詳細(xì)介紹

HB-82 BB7.2小鼠 雜交瘤 B淋巴細(xì)胞

ATCC® Number:HB-82™  Price:$399.00
Designations:BB7.2

Depositors:P Parham

Isotype:IgG2b

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))

Morphology:lymphoblast


Source:Cell Type: hybridoma: B lymphocyte;

Cellular Products:immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxic

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HB-82

Comments:Animals were immunized with papain solubilized HLA A2 antigen.

Spleen cells were fused with NS-1 myeloma cells.

Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding. [39935]

BB7.2 and PA2.1 (ATCC HB-117) recognize overlapping epitopes on the alpha 2 domain of HLA-A2. [39936]

The antibody cross-reacts with cells from various primate species.

Tested and found negative for ectromelia virus (mousepox).



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing:Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.



References:950: Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415

32263: Cole DJ, et al. T-cell receptor repertoire in matched MART-1 peptide-stimulated peripheral blood lymphocytes and tumor-infiltrating lymphocytes. Cancer Res. 57: 5320-5327, 1997. PubMed: 9393756

39935: Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229

39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666


ATCC® Number: HB-82™ Price: $399.00

Designations: BB7.2

Depositors: P Parham

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))

Morphology: lymphoblast



Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxic

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Comments: Animals were immunized with papain solubilized HLA A2 antigen.

Spleen cells were fused with NS-1 myeloma cells.

Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding. [39935]

BB7.2 and PA2.1 (ATCC HB-117) recognize overlapping epitopes on the alpha 2 domain of HLA-A2. [39936]

The antibody cross-reacts with cells from various primate species.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 950: Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415

32263: Cole DJ, et al. T-cell receptor repertoire in matched MART-1 peptide-stimulated peripheral blood lymphocytes and tumor-infiltrating lymphocytes. Cancer Res. 57: 5320-5327, 1997. PubMed: 9393756

39935: Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229

39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 亚洲伦理片 | 日韩福利影视 | 国产盗摄视频手机在线 | v一本久道久久波多野结衣 v一区二区三区 | 国产av一区 | 狠狠躁天天躁夜夜躁婷婷 | 久久综合欧美成人 | 国产一区二区三区观看 | 亚洲欧洲自拍偷线高清一区二 | 麻豆国产在线精品国偷产拍 | 中文成人在线视频 | 亚洲国产成a人v在线观看 | 成人做爰片免费网站 | 成人区人妻精品一区二区不卡网 | 91成人影院在线观看 | 国产高清在线新超碰在线播放 | 久久久久亚洲av成人网人人软件 | 久久一本一区二区三区 | 九一精品 | 亚洲国产精品一区二区久 | 91日韩一区二 | 性瘾日记qvod | 69久久夜色精品国产 | 性欧美XXⅩ 澳门永久成人免费网站 | 99久久亚洲精品日本无码 | 好硬啊进得太深了A片无码公司 | 天天干天天插网 | 国产看片一区二区三区 | av资源每日更新网站在 | 精品亚洲麻豆1区2区3区 | 久久不卡国产精品无码 | 国产日韩ai换脸在线第一页 | 国产精品综合久成人 | 国产麻豆精品久久久久av | 加勒比heyzo高清无码中文 | 国产又色又爽又黄刺激在线视频 | 国产大学生无码视频在线观看 | 人妻无码专区在线视频 | 91精品自拍视频在线观看 | 18精品久久久无码午夜福 | av高清 |